골막기원세포의 조골세포로의 분화과정에서 염증성 사이토카인의 효과

박봉욱; 최문정; 하영술; 조희영; 김덕룡; 김욱규; 김종렬; 변준호

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골막기원세포의 조골세포로의 분화과정에서 염증성 사이토카인의 효과
Evaluation of osteogenic activity of periosteal-derived cells treated with inflammatory cytokines

대한구강악안면외과학회지 2010년 36권 5호 p.341 ~ 345

박봉욱, 최문정, 하영술, 조희영, 김덕룡, 김욱규, 김종렬, 변준호,

소속 상세정보

박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 구강악안면외과학교실
최문정 ( Choi Mun-Jeoung ) - 경상대학교 의학전문대학원 구강악안면외과학교실
하영술 ( Hah Young-Sool ) - 경상대학교병원 임상의학연구소
조희영 ( Cho Hee-Young ) - 경상대학교병원 임상의학연구소
김덕룡 ( Kim Deok-Ryong ) - 경상대학교 의과대학 생화학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김종렬 ( Kim Jong-Ryoul ) - 온종합병원
변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 구강악안면외과학교실

KMID : 0355620100360050341

Abstract

Introduction: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells.
Materials and Methods: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco’s modified Eagle’s medium (DMEM) medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-αwith different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1βwith different concentrations (0.01, 0.1, and 1 ng/mL) were added.
Results: Both TNF-αand IL-1βstimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-αand IL-1βincreased the level of ALP expression in a dose-dependent manner. Both TNF-αand IL-1βalso increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells.
Conclusion: These results suggest that inflammatory cytokines TNF-αand IL-1βcan stimulate the osteoblastic activity of cultured human periosteal-derived cells.